Comparison of the weight-decreasing effects of

different herbs with a mixture of herbal extracts

exerting a probable synergistic effect.

A B S T R A C T INTRODUCTION.

The aim of the present study was to elucidate whether an interaction between different herbal extracts (green tea, Coleus forskohlii, yerba maté, Betula alba) can take place and whether their combination could be more effective in terms of reducing body weight gain than each of the extracts alone. MATERIAL AND METHODS. Sixty rats were treated with different herbal extracts for 12 weeks. Their body weight and food intake was measured weekly. RESULTS. The mixture of herbs leads to a significantly lower body weight increase and lower food intake. The effect of separate herbs was found to be relatively small, a significantly higher weight-increase inhibiting effect was found when using a mixture of herbal extracts.

CONCLUSIONS. A synergic interaction between the components of a herbal mixture is suggested as a possible explanation for the above findings. The probable working mechanism can involve the different modes of action described for the components of the mixture of herb extracts. These mechanisms can involve a decrease in noradrenaline breakdown and an increase in thermogenesis, together with an antioxidative effect and suppressive effect on NFkB activity. The inhibition of adipocyte proliferation and an anorectic effect influencing food intake have also been described for the ingredients included in the mixture. It is suggested that the mixture of different plant extracts can exert several of these effects working in synergy. Key words: obesity, green tea, Coleus forskohlii, yerba maté, Betula alba Endokrynologia, Oty?o?? i Zaburzenia Przemiany Materii 2008, tom 4, nr 4, 137–142 Endokrynologia, Oty?o?? i Zaburzenia Przemiany Materii 2008, tom 4, nr 4, 137–142

Introduction

Obesity is increasing at an alarming rate in most countries, even those whose populations were previously known to be suffering from malnutrition. In the wake of obesity a dramatic increase in the incidence of type 2 diabetes has been observed worldwide. During the second half of the 20th century the rate of obesity increased between fivefold and tenfold. Despite intensive campaigns from medical communities and the introduction of low-fat food products, half of the adult population worldwide are obese or overweight. The World Health Organization is predicting that by 2020 two thirds of the global disease burden will be attributable to chronic diseases associated with obesity [1]. Altering dietary habits is the cornerstone of weight-loss therapy. However, lifestyle interventions aimed at reducing energy intake and increasing physical activity show rather disappointing results, since up to 90% of subjects will eventually return to their initial body weight [2–5]. Compliance with conventional weight management programmes, enriched by popular food supplements advertised intensively as slimming aids, are notoriously poor and the improvements in weight reduction promised in advertisements are not scientifically verified [6, 7]. Apart from cannabinoid receptor blockers [8], most of the slimming preparations on the pharmaceutical market act either through stimulation of thermogenesis [9] or through inhibition of lipase activity (Xenical) [10, 11]. Our goal was therefore to find natural herbal extracts with similar properties. In the scientific literature we found reports describing a thermogenetic effect produced by green tea extract [12, 13], Coleus forskohlii [14–17 and yerba maté [18–22]. There were also reports of an inhibitory effect on lipase activity linked to yerba maté [23] and epigallocatechin gallate (EGCG) [24], which is the main component of green tea. In addition, the polyphenolic and flavonoid components of different herbs show powerful anti-inflammatory effects [25]. A mild inflammatory condition is a characteristic feature of obese humans and rodents fed a highfat diet and is a cause of metabolic syndrome. The aim of the present study was to elucidate whether a synergistic or additive interaction between different extracts can take place and whether their combination could be more effective in terms of reducing of body weight gain than each of the extracts alone. Material and methods Animals Female Wistar rats (n = 60) aged 8 weeks were purchased from the Animal House at the Polish Academy of Science. The animals were first acclimatised to their new conditions over a one-week period and so were aged 9 weeks at the start of the study. During the entire observation period the subject animals were kept under a constant 12/12 light-dark cycle (light from 8.00 am to 8.00 pm) in a room with controlled temperature and humidity. The animals were treated according to the guidelines of the Animal Center at the Polish Academy of Science, Warsaw, Poland. The experimental protocol was approved by the ethical committee for animal studies at the Polish Academy of Science. Study Procedures The rats were randomly divided into five different treatment groups (n = 12 per group). Their body weights varied at the start of the study from 148 g to 209 g with a mean of 173 g in spite of being littermates of the same age. The rats were weighed, as was the amount of food consumed, once a week. The animals had free access to food and water and were given herbal extracts by oral gavage of 0.3 ml volume with their daily dose of test material. This was dissolved in distilled water with the addition of 0.005 g glucose per 1 ml water to improve the taste and for better acceptance of the substances administered. The amounts of herbal extracts were calculated from doses used in humans (Table 1). Test Material Preparation The solutions of all the extracts were produced at the Central Animal Feed Laboratory in Warsaw, Poland. A sufficient amount of test material was dispensed from its original container into an appropriate amount of water. A stir bar was added, and the test material was maintained on a magnetic stir plate. As solutions in these storage conditions (about 23°C) were unstable, they 139 www.endokrynologia.viamedica.pl Marcin Krotkiewski, Rados?aw Janiak, Herb extracts and weight regulation had to be prepared once per week. All solids were diluted in distilled water. After preparation the solutions were exposed to UV radiation. Statistical Analysis Results were analysed according to the ANCOVA model with baseline weight as a covariate. Means were considered significantly different at p < 0.05. Results The average weights of the rats in the different treatment groups are presented in Table 2. The average baseline weights differed between treatment groups, as the randomisation caused variations in the average start weights between different cages and different groups. Table 2. Rat body weight before and after 12 weeks of supplementation (EGCG = green tea extract) Sample N Weight Mean SD SEM Min Max Variance Median XSIII before 186.25 12.836 3.705 165 209 164.75 185.0 12 after 246.08 11.164 3.223 230 268 124.63 245.5 D after–before 59.83 8.441 2.437 49 75 71.24 59.5 Yerba before 168.17 10.161 2.933 148 189 103.24 169.0 Mate 12 after 239.17 14.096 4.069 216 264 198.70 236.5 D after–before 71.00 7.373 2.128 62 85 54.36 70.5 Coleus before 175.00 8.442 2.437 162 190 71.27 175.5 Forskohlii 12 after 253.20 12.912 3.727 232 272 166.73 256.5 D after–before 78.20 10.496 3.030 64 94 110.17 74.5 Betula before 174.33 7.912 2.284 156 185 62.61 176.0 Alba 12 after 251.00 12.714 3.670 218 264 161.64 254.0 D after–before 76.67 8.606 2.484 55 86 74.06 78.0 Green tea before 162.33 5.416 1.563 154 173 29.33 162.5 12 after 236.25 10.010 2.890 223 260 100.20 233.0 D after–before 73.92 7.477 2.158 63 87 55.90 73.5 SD — standard deviation Table 1. Calculations of doses used Substance Human average Rat dose dose per 24 h* per 24 h* Green tea extract 1600 mg 5.33 mg (35% EGCG) Coleus forskohlii 50 mg 0.17 mg Yerba Maté 150 mg 0.5 mg Betula alba 150 mg 0.5 mg Mixture of the above 1950 mg 6.5 mg herbal extracts (XSIII) *An average weight of 75 kg for humans and 250 g for rats was used The rats were weighed once a week for 12 weeks. Figure 1 presents the changes in rat body weight over time during the course of the study. The mean baseline body weights of the different treatment groups were statistically different, the highest being in the group receiving XSIII and lowest in the group receiving green tea extract. Statistical analyses therefore took into account these differences. The increase in weight for each group is presented in Figure 2. Treatment with XSIII was shown to result in a lower weight gain of 32.12% than treatment with Coleus forskohlii, which was associated with a gain of 44.68% (p < 0.0001), Betula Alba a gain of 43.98% (p = 0.0004) and green tea extract a gain of 45.54% (p = 0.0228). In addition, there was a clear tendency towards a lower weight gain in the group treated with yerba maté 42.22% than the XSIII group (p = 0.0753). There were no statistically significant differences between the other treatment groups. The amount of food ingested by the rats in each treatment group was recorded weekly. The mean food intake per week is presented in Figure 3. Food intake was significantly lower in the XSIII group than the groups receiving yerba maté (p = 0.0058), Coleus forskohlii (p = 0.0036) and Betula alba (p = 0.0012), but not significantly different from the group receiving green tea extract (p = 0.1798). Discussion The results described in our study demonstrate a superior effect on the inhibition of weight gain in rats from a mixture of different herbal extracts than from 140 Endokrynologia, Oty?o?? i Zaburzenia Przemiany Materii 2008, tom 4, nr 4 www.endokrynologia.viamedica.pl each of the herbs tested separately. Whether this depends on synergism or on an additive effect cannot be decided definitively. According to a publication by Dulloo et al., the combined effect of green tea extract and caffeine is higher than if each of them is given separately [12]. This observation resembles our findings, as the effects of both green tea extract and yerba maté were found to be lower when given separately than in the herbal mixture XS III. This could indicate a synergistic effect of the green tea extract, yerba maté, Coleus forskohlii and Betula alba on the metabolic processes. Four different mechanisms could theoretically be attributed to this action: a thermogenic effect achieved in different ways by different herbs, an inhibition of li- Figure 1. Body-weight increases in percentages in different groups of rats receiving different herbal extracts for twelve weeks (EGCG = green tea extract) Figure 2. Mean change in body weight after 12 weeks’ follow-up in different groups of rats supplemented with different herbal extracts (Means ± SE). Groups that are significantly different from XSIII are denoted by * (EGCG = green tea extract) Figure 3. Mean food intake in different groups of rats on a diet supplemented with different herbal extracts (Means ± SE). Groups that are significantly different from XSIII are denoted by * (EGCG = green tea extract) pase activity followed by impaired absorption of fat, an inhibitory effect on the proliferation of fat cells or reduced food consumption. The possible thermogenic effect can be due to several different mechanisms. All involve cyclic AMP (cAMP) metabolism. Coleus forskohlii activates adenylate cyclase and the cAMP-dependent protein kinase (AMPK). Yerba maté has a high chlorogenic acid (CGA) content and an important group of biologically active dietary phenols, the best known of which is 5-caffeoylquinic acid (5CQA). Chlorogenic acid is known to inhibit intestinal and hepatic glucose translocase and caffeine itself inhibits phosphodiesterase, an enzyme that degrades cAMP. Finally green tea extract inhibits COMPT, an enzyme that degrades norepinephrine, thus contributing to a higher norepinephrine content [12]. All these additive or synergistic effects result in elevated cAMP, which in turn affects a battery of receptors, enzymes, and signalling proteins, resulting in an increased mitochondrial b-oxidation of fatty acids. Cyclic AMP is also involved in increased synthesis of adiponectin and UCP proteins, both influencing thermogenesis (26-31). A similar mechanism has recently been proposed to explain the weightdecreasing effect of green tea extract when combined with exercise [32]. Green tea and its main flavonoid, epigallocatechin gallate, green tea extract, have been shown to reduce body weight in experimental animals [33–37] and in humans [12, 13, 38–43]. Treatment with green tea rich in green tea extract combined with caffeine was found to result in a significant increase in 24 h energy expenditure when measured in 10 healthy men in a respiratory chamber. The concomitantly observed decrease 141 www.endokrynologia.viamedica.pl Marcin Krotkiewski, Rados?aw Janiak, Herb extracts and weight regulation The administration of XSIII to rats in our study led to a lower food intake as compared to yerba maté, Betula alba and Coleus forskohlii. Decreases in appetite and food intake have previously been reported for yerba maté [20], as well as for caffeine [51–53] and also for intraperitoneally administered green tea [34]. Recent reports could not confirm this particular observation [37, 54] and the discrepancy might be explained by the low bioavailability of catechins [37, 55, 56]. While we could not find any significant difference in food intake between rats fed XSIII and those on green tea extract, there was a significantly lower food intake in this group when compared with the groups receiving Betula alba, Coleus forskohlii and yerba maté. This observation seems to be in agreement with the findings of Murase on the anorexic effect of green tea extract [57]. Although, we do not feel that this mechanism could entirely explain the differences observed in weight gain, it could probably be treated as a plausible contributing factor. Shortly after the termination of the preparation of this manuscript came the publication of a human study by Belza et al. [39] reporting increased thermogenesis and a reduction in fat mass after an 8-week administration of bioactive ingredients with green tea extract as the main component. The results are thus confirmatory to our findings; the authors also discussed the synergistic effects of green tea extract with other ingredients. Taken together, the results of our study and the very recent study of Belza may indicate that there is a place for a mixture of different natural bioactive food/herbal components to be used as adjuncts in the dietary treatment of obesity. in 24 h respiratory quotient was interpreted as indicating a decreased oxidation of glucose and an increased oxidation of fatty acids [12, 13]. Another reason for the lower weight increase observed in rats given XSIII may be an inhibition of lipase activity. A lipase-inhibiting effect has been reported both for green tea extract [24] and yerba maté [23]. Decreased activity of pancreatic lipase may lead to the presence of partially non-digested and therefore non-absorbed fat in the form of a mixture of triglycerides, diacylglycerols, and fatty acids in the lower part of the digestive tract. Impaired digestion will lead to lower uptake of fat from the digestive tract. The presence of this mixture may induce a stimulation of afferent fibres of the vagus nerve and an increased secretion of gut hormones inducing satiety [44–47]. In particular increased content of fat and its derivates can suppress the secretion of grehlin and increase the secretion of peptide YY, which in turn would lead to a reduced appetite [47]. Herbal extracts have previously been found to inhibit lipase activity both in vivo and in vitro [23,24]. However, the magnitude of this inhibition and the observed increase of fat excretion could not entirely explain the weight-decreasing effect of XSIII observed in our study. The third possible mechanism is the antiproliferative effect exerted by green tea extract and other polyphenols on fat cells [48]. It has been proposed that green tea extract could possibly be used in the prevention of weight gain induced by ligands of PPARg and associated with the administration of antidiabetic preparations belonging to the glitazone group [49, 50]. R e f e r e n c e s 1. Chopra M., Galbraith S., Darnton-Hill I.: A global response to a global problem: the epidemic of overnutrition. Bull World Health Organ 2002; 80: 952–958. 2. Ayyad C., Andersen T.: Long-term efficacy of dietary treatment of obesity: a systematic review of studies published between 1931 and 1999. Obes. Rev. 2000; 1: 113–119. 3. Wylie-Rosett J. Paradigm shifts in obesity research and treatment: introduction. Obes. Res. 2004; 12 (Suppl. 2): 85S–87S. 4. Allison D.B., Saunders S.E.: Obesity in North America; an overview. Med. Clin. North Am 2000; 84: 305–332. 5. Haddock C.K., Poston W.S., Dill P.L. et al.: Pharmacotherapy for obesity: a quantitative analysis of four decades of published randomized clinical trials. Int. J. Obes. Relat. Metab. Disord. 2002; 26: 262–273. 6. Allison D.B., Fontaine K.R., Heshka S. et al.: Alternative treatments for weight loss: a critical review. Crit. Rev. Food Sci. Nutr. 2001; 41: 1–28. 7. Pittler M.H., Ernst E.: Dietary supplements for body-weight reduction: a systematic review. Am J. Clin. Nutr. 2004; 79: 529–536. 8. Bosello O., Carruba M.O., Ferrannini E. et al.: Sibutramine lost and found. Eat Weight Disord. 2002; 7: 161–167. 9. Matias I., Bisogno T., Di Marzo V.: Endogenous cannabinoids in the brain and peripheral tissues: regulation of their levels and control of food intake. Int. J. Obes. (Lond) 2006; 30 (Suppl. 1): S7–S12. 10. Padwal R., Li S.K., Lau D.C.: Long-term pharmacotherapy for obesity and overweight. Cochrane Database Syst. Rev. 2004; CD004094. 11. Kelley D.E., Bray G.A., Pi-Sunyer F.X. et al.: Clinical efficacy of orlistat therapy in overweight and obese patients with insulin-treated type 2 diabetes: A 1-year randomized controlled trial. Diabetes Care 2002; 25: 1033–1041. 12. Dulloo A.G., Seydoux J., Girardier L. et al.: Green tea and thermogenesis: interactions between catechin-polyphenols, caffeine and sympathetic activity. Int. J. Obes. Relat. Metab. Disord. 2000; 24: 252–258. 13. Dulloo A.G., Duret C., Rohrer D. et al.: Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation in humans. Am J. Clin. Nutr. 1999; 70: 1040–1045. 14. Okuda H., Morimoto C., Tsujita T.: Relationship between cyclic AMP production and lipolysis induced by forskolin in rat fat cells. J. Lipid. Res. 1992; 33: 225–231. 15. Hoffman B.B., Chang H., Reaven G.M.: Stimulation and inhibition of lipolysis in isolated rat adipocytes: evidence for age-related changes in responses to forskolin and PGE1. Horm. Metab. Res. 1987; 19: 358–360. 142 Endokrynologia, Oty?o?? i Zaburzenia Przemiany Materii 2008, tom 4, nr 4 www.endokrynologia.viamedica.pl 16. Seamon K.B., Daly J.W.: Forskolin: its biological and chemical properties. Adv. Cyclic. Nucleotide Protein Phosphorylation. Res. 1986; 20: 1–150. 17. Majeed K., Badmaev V., Rajendran R.: Method of preparing a Forskohlin Composition, from Forskohlin Extract and Use of Forskohlin for Promoting Lean Body Mass and Treating Mood Disorders. US Patent Nr 5804, 596 (Sept 8): 1998. 18. Dulloo A.G., Seydoux J., Girardier L.: Potentiation of the thermogenic antiobesity effects of ephedrine by dietary methylxanthines: adenosine antagonism or phosphodiesterase inhibition? Metabolism 1992; 41: 1233–1241. 19. Herbal Medicine Expanded Commission E monographs 2000: Mate. American Botanical Council 2000; 249–252. 20. De Pasquale C.: Double-blind controlled clinical study of Mate in subjects placed on low-calorie diet. Clin. Dietol. 1991; 18: 27–38. 21. Verdegem P., Shin J., Johnson M.: A novel nutritional supplement combining Yerba Mate, caffeine and black tea increases energy expenditure. Int. J. Obes. (Lond) 2004; 28: S1, S150. 22. Martinet A., Hostettmann K., Schutz Y.: Thermogenic effects of commercially available plant preparations aimed at treating human obesity. Phytomedicine 1999; 6: 231–238. 23. EFLA®920 Green Mate Special Extract: New findings in weight loss: lipase inhibition a possible explanation. Information Sheet. Frutarom Switzerland Ltd. Pharma Division 2004. 24. Juhel C., Armand M., Pafumi Y. et al.: Green tea extract (AR25) inhibits lipolysis of triglycerides in gastric and duodenal medium in vitro. J. Nutr. Biochem. 2000; 11: 45–51. 25. Santangelo C., Vari R., Scazzocchio B. et al.: Polyphenols, intracellular signalling and inflammation. Ann. Ist. Super Sanita 2007; 43: 394–405. 26. Matejkova O., Mustard K.J., Sponarova J. et al.: Possible involvement of AMP-activated protein kinase in obesity resistance induced by respiratory uncoupling in white fat. FEBS Lett. 2004; 569: 245–248. 27. Puigserver P., Spiegelman B.M.: Peroxisome proliferator-activated receptor- gamma coactivator 1 alpha (PGC- 1 alpha): transcriptional coactivator and metabolic regulator. Endocr. Rev. 2003; 24: 78–90. 28. Zhang Y., Castellani L.W., Sinal C.J. et al.: Peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha) regulates triglyceride metabolism by activation of the nuclear receptor FXR. Genes Dev. 2004; 18: 157–169. 29. Ouchi N., Kihara S., Arita Y. et al.: Adiponectin, an adipocyte-derived plasma protein, inhibits endothelial NF-kappaB signaling through a cAMP-dependent pathway. Circulation 2000; 102: 1296–1301. 30. Prusty D., Park B.H., Davis K.E. et al.: Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma (PPARgamma ) and C/EBPalpha gene expression during the differentiation of 3T3-L1 preadipocytes. J. Biol. Chem. 2002; 277: 46226– 46232. 31. Linhart H.G., Ishimura-Oka K., DeMayo F. et al.: C/EBPalpha is required for differentiation of white, but not brown, adipose tissue. Proc. Natl. Acad. Sci. USA 2001; 98: 12532–12537. 32. Murase T., Haramizu S., Shimotoyodome A. et al.: Reduction of diet-induced obesity by a combination of tea-catechin intake and regular swimming. Int. J. Obes. (Lond) 2006; 30: 561–568. 33. Meguro S., Mizuno T., Onizawa K. et al.: Effects of tea catechins on diet-induced obesity in mice. J. Oleo. Sci. 2001; 50: 593–598. 34. Hasegawa N., Yamda N., Mori M.: Powdered green tea has antilipogenic effect on Zucker rats fed a high-fat diet. Phytother Res. 2003; 17: 477–480. 35. Murase T., Nagasawa A., Suzuki J. et al.: Beneficial effects of tea catechins on diet-induced obesity: stimulation of lipid catabolism in the liver. Int. J. Obes. Relat. Metab. Disord. 2002; 26: 1459– –1464. 36. Zheng G., Sayama K., Okubo T. et al.: Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice. In Vivo 2004; 18: 55–62. 37. Klaus S., Pultz S., Thone-Reineke C. et al.: Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int. Obes. (Lond) 2005; 29: 615–623. 38. Hase T., Komine Y., Meguro S. et al.: Antiobesity effects of tea catechins in humans. J. Oleo. Sci. 2001; 50: 599–605. 39. Belza A., Frandsen E., Kondrup J.: Body fat loss achieved by stimulation of thermogenesis by a combination of bioactive food ingredients: a placebocontrolled, double-blind 8-week intervention in obese subjects. Int. J. Obes. (Lond) 2007; 31: 121–130. 40. Kovacs E.M., Lejeune M.P., Nijs I. et al.: Effects of green tea on weight maintenance after body-weight loss. Br. J. Nutr. 2004; 91: 431–437. 41. Yoshioka M., Imanaga M., Ueyama H. et al.: Maximum tolerable dose of red pepper decreases fat intake independently of spicy sensation in the mouth. Br. J. Nutr. 2004; 91: 991–995. 42. Svendsen O.L., Haarbo J., Hassager C. et al.: Accuracy of measurements of body composition by dual-energy x-ray absorptiometry in vivo. Am. J. Clin. Nutr. 1993; 57: 605–608. 43. Astrup A., Toubro S., Cannon S. et al.: Thermogenic synergism between ephedrine and caffeine in healthy volunteers: a double-blind, placebo-controlled study. Metabolism 1991; 40: 323–329. 44. Orr J., Davy B.: Dietary influences on peripheral hormones regulating energy intake: potential applications for weight management. J. Am. Diet. Assoc. 2005; 105: 1115–1124. 45. Neary N.M., Goldstone A.P., Bloom S.R.: Appetite regulation: from the gut to the hypothalamus. Clin. Endocrinol. (Oxf) 2004; 60: 153–160. 46. Wynne K., Stanley S., McGowan B. et al.: Appetite control. J. Endocrinol. 2005; 184: 291–318. 47. Feinle-Bisset C., Patterson M., Ghatei M.A. et al.: Fat digestion is required for suppression of ghrelin and stimulation of peptide YY and pancreatic polypeptide secretion by intraduodenal lipid. Am. J. Physiol. Endocrinol. Metab. 2005; 289: E948–E953. 48. Lin J., Della-Fera M.A., Baile C.A.: Green tea polyphenol epigallocatechin gallate inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes. Obes. Res. 2005; 13: 982–990. 49. Wang Y., Wolfram S., Preller M. et al.: Green tea catechin, EGCG (teavigo) regulates adipogenesis and prevents TZD-induced weight gain in DB/DB mice. Int. J. Obes. 2006; 28: S1, S90. 50. Wolfram S., Raederstorff D., Wang Y. et al.: TEAVIGO (epigallocatechin gallate) supplementation prevents obesity in rodents by reducing adipose tissue mass. Ann. Nutr. Metab. 2005; 49: 54–63. 51. Acheson K.J., Zahorska-Markiewicz B., Pittet P. et al.: Caffeine and coffee: their influence on metabolic rate and substrate utilization in normal weight and obese individuals. Am. J. Clin. Nutr. 1980; 33: 989–997. 52. Acheson K.J., Gremaud G., Meirim I. et al.: Metabolic effects of caffeine in humans: lipid oxidation or futile cycling? Am. J. Clin. Nutr. 2004; 79: 40– –46. 53. Johnston K.L., Clifford M.N., Morgan L.M.: Coffee acutely modifies gastrointestinal hormone secretion and glucose tolerance in humans: glycemic effects of chlorogenic acid and caffeine. Am. J. Clin. Nutr. 2003; 78: 728–733. 54. Choo J.J.: Green tea reduces body fat accretion caused by high-fat diet in rats through beta-adrenoceptor activation of thermogenesis in brown adipose tissue. J. Nutr. Biochem. 2003; 14: 671– –676. 55. Chen L., Lee M.J., Li H. et al.: Absorption, distribution, elimination of tea polyphenols in rats. Drug Metab. Dispos. 1997; 25: 1045–1050. 56. Lee M.J., Maliakal P., Chen L. et al.: Pharmacokinetics of tea catechins after ingestion of green tea and (–)- epigallocatechin-3-gallate by humans: formation of different metabolites and individual variability. Cancer Epidemiol. Biomarkers Prev. 2002; 11: 1025– 1032. 57. Murase T., Haramizu S., Shimotoyodome A, et al.: Green tea extract improves endurance capacity and increases muscle lipid oxidation in mice. Am. J. Physiol. Regul. Integr. Comp Physiol. 2005; 288: R708–715.

Diet To Lose Belly Fat

It is false to assume that a diet to lose belly fat should rely mainly on fruits and vegetables; dairy products, lean meat and seeds also have their role to play here. Fat storage is usually favored by the consume of certain foods that impair the metabolism. Commercials often persuade people to take up a number of  diet to lose belly fat solutions. I tell you that you need to make informed decisions that suit your individuality, health condition and needs. Plus, you should never neglect personal preferences.

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A diet to lose belly fat is one that aims at achieving overall body fitness, and its success depends on several factors.

The diversity of foods matters!

Some restrictive diets allow you to eat only chicken soup, apples and yogurt for a whole month. There are many negative consequences to food restrictions. This is not a diet, this is torture! No wonder you can’t lose fat!

Eat five or six meals per day, with small portions and a diversity of foods. There is an abundance of food recipes available and your choices are virtually countless. Food provides energy and nutrients that you can’t live without, and the absence of these nutrients from the diet will alter your physical condition and your health for the worse.

Training supports diet and vice versa!

No diet to lose belly fat will work unless it combines training with diet. A healthy life depends on the quality of the lifestyle, and most people ignore this very important aspect of their existence.

Abdominal fat usually affects people with a sedentary lifestyle who also make poor food choices. You can get in shape by practicing a sports activity you take pleasure in. You can commit to a sport, a regular gym routine, a dance class or anything else that works your muscles and burns calories.

Overweight goes hand in hand with bad habits!

Alcohol is the main enemy of any diet to lose belly fat. Beer is the main culprit for abdominal obesity, which is why we call it ‘beer belly’. Beer has lots of calories and it also interferes with the functions of the pancreas and liver, which are responsible for the secretion of insulin. There is a close connection between diabetes and abdominal fat. Watch out!

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Quit drinking!

Quit smoking!
Sleep at least eight hours per night!
Do physical activities!
Think positive!
Eat healthy food!

…And you’ll enjoy a slender body, a great waist line and very good health!

  Best Diet Pills Review

Gaining weight definitely appears in the list of people’s concerns nowadays.  As such, different options are made available.  . Although modern ways of burning fat are being made available, the natural way is still best.   However, those lazy people who cannot take much time in the gym and cannot resist personal cravings, they choose the fastest way to lose weight through liposuction and diet pills, but they do not exactly know what are the health risks that can pose detrimental effects to their health. If you really want to lose weight and maintain a healthy lifestyle, why not try the natural way? The satisfaction of losing weight in the usual manner is usually greater than that of the artificial.

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Burning fat naturally and healthily still maintain long-term results.  Choosing the artificial way over the natural one may cause more adverse long-term effects.   Moreover, our body’s metabolism is basically related to both losing weight naturally and the other way. If a person chooses to lose weight the natural way, his/her metabolism would reach its peak making the body burn a bigger amount of fat. When one chooses the artificial/modern way, such as drinking pills or undergoing through liposuction, the body’s metabolism would respond the other way around without burning any amount of fat.   So, which do you choose?  The option that allows you to preserve what you’ve been working for although the results can be first seen a little longer.   Or to a fast method with health risks attached to it?  Regular exercise and the consumption of the right kind and amount of food allow the body to work at its fullest potential in burning fat.

The best decision is one preferring the healthier way. Although you’ll have a hard time in reaching your goal by using the natural way, the results of this will be preserved for a longer period. This write-up is definitely for you, my friend, if you are very much conscious of your well-being and you would rather choose the natural way over the artificial one. Here are some tips that would assist you in reaching your “healthy” goals:

DIET

• The idea of not eating is one of the most frequent blunders in losing weight.   Because some people do not eat properly, they are more prone to not reaching the weight that they want.   Eating large meals is less advisable than eating smaller fractions often in a day.   This little switch to a healthier eating habit will allow your system to work at its fullest and will result to a bigger chance of losing more weight.
• Eat a balanced diet.  Although you are dieting, it is still necessary for your body to get all the important nutrients for your body.  A diet which contains proteins, vitamins, minerals, carbohydrates and healthy fats will allow you to reach your goal.   It is advised that you should be consuming more protein during your diet to compensate for the calories being deprived.  Proteins are essential to conserve muscles, healthy fats, and good carbohydrates that would allow you to get through with your daily activities.    A proper balance of these items is essential for any fat loss diet.
•      ). Slash down your carbohydrate intake and increase your protein consumption by avoiding white foods (bread, pasta, white rice).    . Slash out sugars and simple carbohydrates since these can add to your weight.  So avoid as long as you can even if it tastes good.
• Lower down your intake of foods that do not aid you in your process of weight loss.  . If possible, eliminate your consumption of sugary foods such as, cookies, chips, crackers, chocolates, etc.    Gobble on whole grains, fresh vegetables, fruits and lean meats rather than junk foods.  Be smart and conscious about what you put into your body. Instead of indulging into foods that cause you to gain more weight, eat the ones that are more nutritious as this reflects your personality.
• To speed up your fat burning system, drink 8 to 10 glasses of water daily and avoid sodas.  Gulping in H2O allows your body to flush down the toxins.   Our bodies are mostly water, so keeping a constant supply is critically important. In this way, we are being prevented from eating more.

EXERCISE

•   Start a physical activity such as an aerobic exercise program.  Aerobics increases your body’s ability to burn more calories. Begin with an easy set of aerobics routine if you are overweight.   Jumpstart your day by doing at least 30 minutes of walking.
•  You may perform weight-bearing workouts thrice a week.  This activity strengthens the bones and enhances the creation of muscle tissues.    The metabolism of our body as well as its fat burning process is accelerated due to the added muscle tissues.

• Do not forget to stretch after workouts to increase flexibility and help prevent muscle trauma and injury to your muscles.

• Workouts allow you to look and feel fabulous by burning body fat, making you look younger and enhancing your system’s defense. Remember, the more muscles you get involved in your workout, the more fat you’ll burn.  A set of workout activities must include cardiovascular activities and resistance training exercises.

FAT BURNING FOODS

In the process, these foods use up a large amount of energy but preserves enough for the body.    These consist the following:

• Egg
• Oatmeal
• Lean meats
• Fish
• Apples
• Beans
• Dairy products (low fat)
• Olive oil
• Whole grains
• Carrots
• Chicken

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If you have belly fat and you are complaining that it is tough to cut it out, worry no more becauseright now you can find out some  useful guidelines on how you can manage your issue.   The initial matter that you must do is to understand your problem and settle for that it is indeed leading to you trouble. This is a reality check step.   If you don’t determine it, you could locate it tough to handle it.   Outlined beneath are most of the handy ways on how you can decrease your stomach fat.Examine carefully and consider be aware of most vital reminders.

Eat a balanced diet

Your belly fat came about from consuming high ldl cholesterol foods. If you are fond of eating chocolates, cakes, ice cream, and french fries, afterwards you can produce it easily.   Yet another trigger is too much intake of alcohol, such as beer. Which is why your belly fat is from time to time known to as a “beer stomach.”

You want management what you eat, but you do not want starve. Simply eat in moderation and be sure that you eat a well balanced diet plan. Which is, you should cope far more with veggies and fruits, instead of hamburgers, hot dogs, and sweets.

Physical exercise every day

There are unique sorts of exercises, and most of them are meant to reduce the size of the stomach. Sit-ups are the best kind of stomach physical exercise because they generate your stomach muscle tissue firm and they get rid of the further fat deposits in which area.

Apart from sit-ups, you can additionally do a lot of jogging, leaping, and weight lifting to reduce your stomach size. Remember that you must sweat quite a few if you want to reallyburn your fats.

Minimize stress

You need to be active, but you could prevent obtaining too stressed.  Your body cannot burn the fat deposits in your body if you often live a taxing lifestyle.  Furthermore, you can possess a tendency to eat a lot more if you are  stressed. You must also get a good overall amount of rest so that your thoughts can concentrate on your aim to lose individuals excess pounds on your stomach.

These 3 approaches to shed stomach fat rapid are verified effective by people who have attempted them. You too can realize success in your venture if you put many difficult perform into it.   Are you seeking for the greatest way to Lose Belly Fat?

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Lose Belly Fat Fast

To Lose belly fat fast involves a simple mathematical formula: if you eat more calories than what your body needs, the extra calories are stored as fat. This simple formula, however, becomes difficult for people who love eating and requires other effective means to achieve fast fat loss. Meanwhile, those who are disciplined with their eating need to follow only two things to lose weight fast: eat fewer calories or use more calories than what you eat.

Burn belly fat fat fast does not necessarily mean spending all day in the gym. Aside from gym workouts, you can also involve yourself in daily chores, yard work, and brisk walking as forms of physical activity that allow your body to break into sweat. According to research, the human legs have the largest muscle groups, thus using your legs to burn belly fat quickly is very effective. Adding at least 30-45 minutes of physical activity to your day is a great way to lose tummy fat fast. Any form of physical activity that raises your hear rate can translate to dramatic weight loss. Research has proved that even if you do not change anything in your diet, you can lose two to four pounds of weight in one week by simply involving yourself in regular strenuous activities. However, you have to make sure that the physical activity that you do can elevate your heart rate so that your fats will be burned.

You should also cut down on your intake of liquid calories as part of the fat burning tips that you apply to yourself. Liquid calories refer to soda and fruit juice. Regular intake of liquid calories can increase your waistline measurement. Completely removing these from your diet can shed at least one to three pounds in just seven days. This is because a can of regular soda contains 150 – 200 calories while a cup of apple juice has around 120 calories. Water, a zero-calorie drink, is the best replacement for liquid calories. In fact, water is perhaps the best fat burner( reduSlim.com) available since it hydrates your body and helps you burn more calories by increasing your rate of metabolism.

For additional but healthy flavor, you can choose to squeeze in fresh lemon or lime to your water. Another healthy alternative with zero calories is green tea. You should also fill up on fiber if you want to lose belly fat quickly.

A great way to lose belly fat fast  is to replace processed foods with fiber-rich whole grain foods. So, instead of white bread, make your sandwiches using whole grain or whole wheat bread. For your dinner, opt for brown rice instead of white rice. This is because fiber-rich foods like whole grains make you feel full longer and help you eat less through out the day.

Ressources below.

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Buy Diet Pills Online

Whenever you prepare to purchase fat burners on-line you are certainly not only saving time and energy but you can also acquire access to critical information regarding the goods you want to purchase. When you pay for slimming capsules online the significant variables that each and every purchaser. You should verify the organization or product or service.  The particulars relating to side effects , the fees. Aside from that you simply also ought to know if the transaction with them on the web is safe and will guard your personalized info.

Before you buy diet pills online, do some analysis to find out which products will be the best for your  and which fits your spending budget. You can also consult a doctor for assessment. Whenever you make a obtain, you ought to establish 1st which form of pill you require. You can find capsules that enhance metabolic rate, suppress hunger, calorie-busting, weight removing and excess drinking water eliminating. Slimming capsules that raise metabolic rate burn fat quickly. Appetite suppressant tablets lessen the urge to eat and are excellent for folks who come across it challenging to control eating. Some  powerful suppressants  are Phentermin and Phentramin-D. Calorie-busting pills are for people who take in excess of every day consumption and individuals who wish to be free  from obesity. If you would like tablets that act as cleansing agents, you must find weight loss supplements on-line which can be fat-eliminating.

Once you buy diet pills online, you really should know essentially the most well-liked types. Phentermine 37.5 is one of the most well-liked Phentermine dosage prescription. It can be sold as Phentermine 37.5 mg tablets and it blocks neurotransmitters accountable for sending the message for your brain that you just are hungry. It is for persons who aim to shed a lot more than 15 pounds. Phentramin-D is often a slimming pill more powerful than Phentermine and you also do not need to have  a prescription to purchase this merchandise. But it remains a chemical pill. You need to buy diet pill online that are more natural.

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Herbal Diet Pills

So many people are dealing with a huge weight issues, constantly dieting to no avail. While you may have been successful at shedding some pounds originally, you probably were unable to keep them off on a long-term basis. Thankfully, there are herbal diet pills to help shed pounds and maintain a healthy weight.

A lot of the problem has to do with the way that people live and eat. The Western diet has become extremely popular, reaching all around the world. More and more of us are looking for quick fixes for fast dinners that are processed, refined, and prepared with all kinds of unhealthy ingredients that are full of fat, because we’re very busy. Couple that with the fact that many of these quick fixes are absolutely delicious, make it really hard on everyone.
That results in weight gain. It’s important that people look at the negative aspects that lie within the Western diet. Changing that diet is imperative in order to live a longer and healthier life. It’s no wonder that life-threatening conditions are popping up all over the place, including diabetes, increased heart disease, and such. A lot of it is also due to the fact that we rely on our cars to get around. We just don’t move about enough anymore. The vast majority is working in a seated position, barely moving all day long. Then, they go home and lie on their couches, watching television or gaming.
So a remedy must be found. Thankfully, the herbal diet pills can get the results you want. These herbal diet pills will see you shedding pounds after pound gradually, until you reach your goals. But, it is truly effective when you make some changes with regards to the way you eat and by exercising.
The herbal diet pills will help to increase the metabolism in your body, thereby burning more calories quicker than ever. They will suppress your appetite, help you feel full faster, and provide you with a lot more energy.
This way, you’ll reduce your cravings and avoid eating in excess. You’ll also be able to reduce the absorption of fat by taking these supplements. There are many people who don’t overeat and yet still gain weight. A lot of times, this is the result of a very slow metabolism, or because of physical limitations, or because they are living a sedentary lifestyle.
Unfortunately, when people put on weight, they try anything to drop the excess pounds. But there is no magic pill or a magic solution. You need to work at it and you need to be true to your efforts. So, you have to make a commitment that you will improve your diet, move around a little more, and supplement your meals.
Only in this way will you be able to reap the rewards of a well functioning and good-looking body. These herbal diet pills, or supplements are full of good and natural ingredients that will not be harmful to you, nor will you form an addiction to them. Green tea , is one of the ingredients, that you may or may not have already heard us. It isthis that will suppress your appetite and leave you feeling fuller for longer.

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Herbal Weight Loss Pills

There have been many research studies out lately that say the world’s human inhabitants are obese. This is due in part to a number of things but poor diet and portion control are major factors in all parts of the world. People are over eating and not eating the right things to maintain a healthy lifestyle and body image. Many people are trying the weight loss properties in herbal weight loss, pills, liquids and powders in order to ward off the excess fats their bodies are collecting from over eating.
There are many different herbs that are available through a health food store, either online or around the corner, to help an individual gain control over their eating habits and come up with a better solution to losing weight. Weight gain is often easy; taking it off is the hard part. There is no miracle cure or magic pill or powder that one can take to make the pounds slip away over night. We all wish there was. There are however, herbal weight loss pills that will help to curb a person’s appetite so that they are less hungry before a meal. These herbal supplements help to fill a person’s stomach and trick the brain into thinking they are full. This turns off the receptors that tell the mouth to keep eating which is the main cause of weight gain.
The herbal weight loss pills come in a variety of styles for everyone’s tastes. There are the slimming green tea’s that are very popular and seem to work very well for some people. People who find it easier to drink a liquid such as a green tea with an herbal slimming aide inside before each meal will see the pounds slowly coming off, while others prefer a more natural approach. There are herbs that are ground to a fine powder or herbs that are sprinkled on top of a meal such as those that look like oregano or other spices. In addition they not only help a person loose weight, they add flavor to a meal.
Some of the herb capsules are taken three times a day, with every meal. Others are taken just once a day in the morning or at bedtime. There are still other types of the slim down herb that is in powder form and could be mixed into a shake, coffee, juice or soda and taken before a meal or as an afternoon snack. This would curb a person’s appetite later in the day and into the evening which is when a lot of people tend to over eat.
Herbs are all natural and people like this better than some of the synthetic brands. These herbs are grown and processed all over the world and are deemed safe by all governing bodies of vitamins and supplements in the US and other countries. These herbs will not make a person jumpy or jittery and in some cases, they will give a person more energy then they had before.
Of course herbal weight loss pills are not the only way to lose weight. It should be combined with a healthy diet and exercise plan. A good lifestyle change is what has always worked for the thousands of people who loose weight and consistently keep it off. And always check with a doctor before starting any type of weight loss plan, even the natural kind, to make sure a person is healthy and there are no other problems.
There are some people who have lost weight with herb weight loss pills or teas and they will take one or two days a week and use the supplements again as a way of maintaining their weight loss goal. This helps to keep them motivated and on track to keep the extra pounds off and not rebuild their old body of fat they want to leave behind. Some people say loosing the weight is the hardest thing, other say keeping it off is harder and still other say that once they loose their weight, they will never go back to being heavy again.

There are many different types of herbal weight loss pills for someone to take to help them lose weight. With a little trial and error, an individual could find the one that works best for them. There are slimming teas, powders, pills and liquids depending on what a person’s taste is. Whichever type of herb formula a person chooses, they should give it at least a week to get in their system and start working before giving another type a try.

Below some herbal weight loss pills that have been reviewed and proven to work well.

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Of course, fast fat loss is a goal many people share. Take an honest look at your diet before buying a weight loss supplements. If your diet is full of things like saturated fats or high calorie foods. Tracking your calories will help you know that you are eating a reasonable amount. And you need to make sure these calories are coming from healthy foods. High fructose corn syrup and other unnatural ingredients should be avoided as much as possible. For a good source of vitamins and nutrients, you can’t go wrong with fruits and vegetables. Eating this way will help your body make the most of what you give it and also if you decide to use a weight loss supplement you will get better results out of it.

Even more effective than  just weight loss supplements is a good exercise routine. Here’s the truth: very few people actually enjoy working out. Even with good music and comfortable shoes, working out is often tedious and many people find it boring. It does, however, release a nice rush of endorphins that you won’t get from hardly anything else.

Green tea has been put to use for a bunch of different things. It isn’t just used for weight loss supplements. Fat absorption from the body is supposed to be decreased with Green tea.

There are plenty of ways to lose weight. But the best way remains eating a healthy diet and exercising regularly. Trying to get rid of excess weight can be hard and sometimes it may seem like you need a little extra help. Using weight loss supplements can be of an aid. But remember to talk to your doctor if you suffer from pre-existing health problems. Below top weight loss supplements we reviewed.

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How To Lose Weight Quickly: Quinoa Super Diet

We have seen many diets throughout the years. A lot promise that you will lose weight quickly, but do they actually work? The Quinoa Super Diet works.

The Quinoa Super Diet is new and is having outstanding results. It hasn’t become popular yet because not many people have heard of Quinoa. Quinoa, a species of goosefoot (Chenopodium), is a grain-like crop raised chiefly for its edible seeds. As a chenopod, quinoa is closely related to species like beets, spinach, and tumbleweeds.

What is quinoa really, and how will it help shed pounds ?

Quinoa (KEEN-WAH) is a super grain and one of the most potent nutritional foods on earth .  It is being considered the new super food. Quinoa is basically a seed. It is used as a grain and substituted for grains because of its cooking characteristics. The quinoa seed is high in protein , calcium and iron , a good source of vitamin E , in addition to containing many B vitamins. It consists of a just about perfect balance of all eight essential amino acids needed for tissue development in human beings. Quinoa has 6 to 7% fat and is loaded with 12 to 18% protein. The fat however is countered by the exceptionally low sodium content and the benefit of starch and fiber. The seeds are gluten-free which makes this a nutritious and flavorful alternative grain for those with diabetes

Quinoa has high amounts of magnesium which helps to relax blood vessels.  It is cholesterol free and is a great option for vegetarians, people with certain food allergies, or anyone looking to increase their intake of high quality protein. It also contains the amino acid lysine, which is essential for tissue repair and growth , and is a wonderful source of iron, copper and phosphorous.

Okay so, we know quinoa is good for us, but how will it help us lose weight quickly?

The Quinoa Super Diet was created to have maximum adaptability which is then able to accommodate different preferences, tastes, and lifestyles. An American woman of average size can lose 7 lbs in 7 days with a man being able to lose even more inside those 7 days.

Most times when you are trying to lose weight , you have to eat tasteless food that makes you want to quit the diet plan sooner.  Quite different from other diet plans this one is mouth-watering so that you enjoy your diet plan. Quinoa can yield many different flavors depending on how it is prepared . The seeds tend to take on the flavors of the liquids that are added to it, like a stir fry only better. People like to cook with chicken or vegetable stock  which gives the seeds a rich flavor. There are a bunch of quinoa recipes on the market to pick from and experiment with.

The best part is that because of its status as a low glycemic  index product it will keep you feeling fuller for longer so you can fight off those cravings oft experienced with other diets. And we all know that to lose weight you have to take in fewer calories that you burn off. So, keeping those craving away is important to all diets .

Quinoa seed can be made into a protein rich, easy to digest, and tasty diet that will melt your fat and lower your weight to give you that perfect body you have always imagined . In addition to that killer body, you will also be healthier and have a greater quality of life.

For more information please visit Quinoa Super Diet Blog